Post-treatments of polydopamine coatings influence cellular response

Polydopamine (PDA) is the final oxidation product of dopamine or other catecholamines. Since first reports PDA coatings starting around 2007, these have been widely studied as a versatile and inexpensive one-step coating option for biomaterial functionalization. The attach to wide range materials can subsequently be modified with biomolecules nanoparticles. However, strong candidate research even clinical use, it important unravel changes in physico-chemical properties cell-PDA interaction function heat sterilization procedures shelf storage periods. Four groups were examined this study: titanium (Ti), PDA-coated Ti samples either stored up two weeks at room temperature heated 121 °C 24 h, respectively. We used X-ray Photoelectron Spectroscopy (XPS), Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) Water contact angle (WCA) characterize chemical composition surface groups. Cell adhesion proliferation was by three different cell types: human primary dermal fibroblasts (hDF), epidermal keratinocytes (HaCaTs) murine preosteoblastic line (MC3T3-E1), Cells cultured on coated 4 3 days 5 days. Both thermal treatment 121℃ h 2 increased amount quinone decreased amine detected XPS ToF-SIMS. Even though reactions WCA coating, we found that post-treatments both hDFs, HaCaTs MC3T3-E1 s compared pristine PDA. This emphasizes importance post-treatment shelf-time coatings.